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KMID : 0370220100540010039
Yakhak Hoeji
2010 Volume.54 No. 1 p.39 ~ p.48
The Simultaneous Analysis of Sennoside A and Rhaponticin in Rhei Rhizoma using Liquid Chromatography-Diode Array Detecter
Kim Ouk-Hee

Kwak Jae-Eun
Chung Sam-Ju
Kim Dong-Gyu
Han Eun-Jung
Han Chang-Ho
Kim Bok-Soon
Choi Byung-Hyun
Kim Min-Young
Abstract
The objective of this study was to analyze sennoside A and rhaponticin simultaneously according to sennoside A of Rhei Rhizoma determination in Korean Pharmacopoeia. solution in KP was compared with methanol which usually used as solution to extract rhaponticin in Rhei Rhizoma. The method was validated through the guidelines of linearity, LOD, LOQ, specificity and accuracy. Two solution weren¡¯t different about validation parameter and passed. So this method were applied to the determination of 6 commercial Rhei Rhizoma samples and 2 samples were suitable for the legal standards.
KEYWORD
Rhei rhizoma, sennoside A, rhaphonticin, HPLC, NaHCO3 solution
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